Search results for "methods [Proteomics]"
showing 10 items of 99 documents
Computational identification of cell-specific variable regions in ChIP-seq data.
2019
ABSTRACT Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is used to identify genome-wide DNA regions bound by proteins. Several sources of variation can affect the reproducibility of a particular ChIP-seq assay, which can lead to a misinterpretation of where the protein under investigation binds to the genome in a particular cell type. Given one ChIP-seq experiment with replicates, binding sites not observed in all the replicates will usually be interpreted as noise and discarded. However, the recent discovery of high-occupancy target (HOT) regions suggests that there are regions where binding of multiple transcription factors can be identified. To investigate these regions,…
Stochastic analysis of dynamical systems with delayed control forces
2006
Abstract Reduction of structural vibration in actively controlled dynamical system is usually performed by means of convenient control forces dependent of the dynamic response. In this paper the existent studies will be extended to dynamical systems subjected to non-normal delta-correlated random process with delayed control forces. Taylor series expansion of the control forces has been introduced and the statistics of the dynamical response have been obtained by means of the extended Ito differential rule. Numerical application provided shows the capabilities of the proposed method to analyze stochastic dynamic systems with delayed actions under delta-correlated process contrasting statist…
Genotoxicity investigations on nanomaterials: methods, preparation and characterization of test material, potential artifacts and limitations--many q…
2008
Nanomaterials display novel properties to which most toxicologists have not consciously been exposed before the advent of their practical use. The same properties, small size and particular shape, large surface area and surface activity, which make nanomaterials attractive in many applications, may contribute to their toxicological profile. This review describes what is known about genotoxicity investigations on nanomaterials published in the openly available scientific literature to-date. The most frequently used test was the Comet assay: 19 studies, 14 with positive outcome. The second most frequently used test was the micronucleus test: 14 studies, 12 of them with positive outcome. The A…
Comparative evaluation of molecular methods for the quantitative measure of torquetenovirus (TTV) viremia, the new surrogate marker of immune compete…
2019
Background Torquetenovirus (TTV) viremia is emerging as a promising tool to assess functional immune competence, to predict posttransplant immune-related complications, and eventually to customize immunosuppression. Methods In this study, 327 blood samples were tested using two real-time PCR (rtPCR) assays both targeted to the untranslated region of the TTV genome. The first assay was an in-house rtPCR developed by our group, the second one was the recently marketed TTV R-GENE assay. Results In the validation study, the TTV R-GENE showed good performances in precision and reproducibility, and sensitivity as low as 12 TTV DNA copies/mL, like previously reported for the in-house rtPCR. The Bl…
Italian multicenter, prospective study to evaluate the negative predictive value of 16- and 64-slice MDCT imaging in patients scheduled for coronary …
2009
This was a prospective, multicenter study designed to evaluate the utility of MDCT in the diagnosis of coronary artery disease (CAD) in patients scheduled for elective coronary angiography (CA) using different MDCT systems from different manufacturers. Twenty national sites prospectively enrolled 367 patients between July 2004 and June 2006. Computed tomography (CT) was performed using a standardized/optimized scan protocol for each type of MDCT system (> or =16 slices) and compared with quantitative CA performed within 2 weeks of MDCT. A total of 284 patients (81%) were studied by 16-slice MDCT systems, while 66 patients (19%) by 64-slice MDCT scanners. The primary analysis was on-site/off…
TWJ-Screen: an isothermal screening assay to assess ligand/DNA junction interactions in vitro
2017
International audience; The quest for chemicals able to operate at selected genomic loci in a spatiotemporally controlled manner is desirable to create manageable DNA damages. Mounting evidence now shows that alternative DNA structures, including G-quadruplexes and branched DNA (or DNA junctions), might hamper proper progression of replication fork, thus triggering DNA damages and genomic instability. Therefore, small molecules that stabilize these DNA structures are currently scrutinized as a promising way to create genomic defects that cannot be dealt with properly by cancer cells. While much emphasis has been recently given to G-quadruplexes and related ligands, we report herein on three…
Cruise passengers' expenditure at destinations: Review of survey techniques and data collection
2020
Spending by cruise passengers constitutes an important contribution to the economy of destinations. The issues related to the analysis of the expenditure have been widely debated for many years both in the scientific literature and in the common political debate. Despite 20 years of empirical research on this topic, not consolidated quantification methods exist to inform the debate. This work offers a brief review of the principal research on cruise tourist spending. It analyses the main characteristics of the surveys, reviews the different methods and techniques employed as well as assesses the main results. The article concludes with a discussion of the studies conducted and identifies fu…
Externalities and article citations: experience of a national public health journal (Gaceta Sanitaria)
2016
Purpose: The purpose of the study was to analyze the determinants of citations such as publication year, article type, article topic, article selected for a press release, number of articles previously published by the corresponding author, and publication language in a Spanish journal of public health. Methods: Observational study including all articles published in Gaceta Sanitaria during 2007-2011. We retrieved the number of citations from the ISI Web of Knowledge database in June 2013 and also information on other variables such as number of articles published by the corresponding author in the previous 5 years (searched through PubMed), selection for a press release, publication langua…
H∞ static output-feedback control design with constrained information for offshore wind turbine system
2013
Abstract This paper deals with H ∞ static output-feedback control design with constrained information for offshore wind turbines. Constrained information indicates that a special zero–nonzero structure is imposed on the static output-feedback gain matrix. A practical use of such an approach is to design a decentralized controller for a wind turbine. This will also benefit the controller in such a way that it is more tolerant to sensor failure. Furthermore, the model under consideration is obtained by using the wind turbine simulation software FAST. Sufficient conditions to design an H ∞ controller are given in terms of Linear Matrix Inequalities ( LMI s ). Simulation results are given to il…
Bibliometric analysis through methodological quality indicators of Spanish education journals indexed in JCR during the three year period 2014-2016
2019
This study carries out a bibliometric evaluation through methodological quality indicators of the scientific production published during the triennium of 2014 to 2016 in the seven Spanish journals of education indexed in the Journal Citation Reports (JCR): Revista de Psicodidáctica; Comunicar; Revista de Educación; Educación XX1; Enseñanza de las Ciencias, Revista Española de Pedagogía; y Porta Linguarum. Through a bibliometric-descriptive methodology, a sample of articles that totals 479 and selected through a non-probabilistic sampling of deliberate type. Overall, the results obtained indicate that the journals evaluated have achieved levels of compliance with the proposed methodological …